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Mouse TNF alpha PicoKine ELISA Kit

2020/4/3 16:38:08 浏览:
  • 【品牌】: 博士德
  • 【産品貨號】: EK0527
  • 【價格】: 1200  元
  • 【規格】: 48T/96T
  • 【貨期】: 現貨
  • 在线订购
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 産品說明
【品牌】: 博士德
【産品貨號】: EK0527
【價格】: 1200  元
【規格】: 48T/96T
【貨期】: 現貨
【儲存溫度】: -20

Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.

典型操作程序:

准備試劑和標准品

每孔添加100ul樣品和標准品,37℃反應90分鍾,不洗。

每孔添加100ul生物素標記抗體,37℃反應60分鍾。

0.01M TBS洗涤3次。

每孔添加100ulABC,37℃反應30分鍾。

0.01M TBS洗涤5次。

7 TMB37℃避光反應30分鍾以內。

加入TMB終止液,讀數。


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